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Image Search Results
Journal: Translational Lung Cancer Research
Article Title: Emergence of a HER2 -amplified clone during disease progression in an ALK -rearranged NSCLC patient treated with ALK-inhibitors: a case report
doi: 10.21037/tlcr.2020.04.03
Figure Lengend Snippet: CNV analysis performed with NGS and ddPCR. (A) Plot of normalized coverage levels is shown. Blue dots correspond to target regions without gene amplification (normal regions), orange dots to potential amplified genes; in particular the arrow indicate HER2 gene amplification; (B) ddPCR confirmation on DNA used for NGS analysis, extracted from tissue biopsy, was performed with CNV Assay for HER2 (dHsaCP1000116) and AP3B1 as recommend reference assay (dHsaCP2500348) with ddPCR QX200 Droplet Reader (Bio-Rad, USA) according to the manufacturer’s protocol; (C) CNV studied in cfDNA retrospectively from disease progression (PD) sample backward. CNV, copy number variation; NGS, next generation sequencing; ddPCR, digital droplet PCR; cfDNA, cell-free DNA.
Article Snippet: DNA was extracted from the liver biopsy and studied by next
Techniques: Amplification, CNV Assay, Next-Generation Sequencing